Anticorps Monoclonal anti-LYN

LYN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C7F2

N° de cat : 60211-1-PBS

Synonymes

JTK8, LYN, Tyrosine protein kinase Lyn

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Galerie de données de validation

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  WB analysis of Jurkat using 60211-1-Ig (same clone as 60211-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60211-1-Ig (same clone as 60211-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60211-1-Ig (same clone as 60211-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LYN antibody (60211-1-Ig, Clone: 3C7F2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60211-1-PBS cible LYN dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LYN Protéine recombinante Ag12723
• Nom complet: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Masse moléculaire calculée: 512 aa, 59 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC075002
• Symbole du gène: LYN
• Identification du gène (NCBI): 4067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

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