Anticorps Polyclonal de lapin anti-Lysozyme

Lysozyme Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (2)

N° de cat : 15013-1-AP

Synonymes

1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM

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Galerie de données de validation

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  WB analysis of human spleen using 15013-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 15013-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 15013-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (Lysozyme antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15013-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 15013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules HL-60, tissu pulmonaire de souris, tissu splénique humain
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

15013-1-AP cible Lysozyme dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Lysozyme Protéine recombinante Ag6917
• Nom complet: lysozyme (renal amyloidosis)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC004147
• Symbole du gène: LYZ
• Identification du gène (NCBI): 4069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Publications

Species	Application	Title
		Cell Chem Biol; Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids.; Authors - Sara H Rouhanifard
human		Arch Toxicol; Extending the viability of human precision-cut intestinal slice model for drug metabolism studies.; Authors - C Biel
mouse	WB	FASEB J; Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.; Authors - Brittany R Jenkins
mouse		Int J Mol Sci; Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.; Authors - Wenyan Yang
rat	IF	Physiol Genomics; The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats.; Authors - Kari Chesney
human		Histol Histopathol; Immunohistochemical study of inflammatory responses in septa arising from type I intestinal atresia.; Authors - Xuelai Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for colon tissue and colon cell line. this antibody is working great and no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and colon cell line (caco2 cells)

FH

Claudio (Verified Customer) (12-15-2021)

The antibody staining did not yield any labelling, though it was tested in paraffin sections so you might get better results with cryostat sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse heart sections