- Phare
- Validé par KD/KO
Anticorps Recombinant de lapin anti-Lamin A/C
Lamin A/C Recombinant Antibody for IF/ICC
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
IF/ICC
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5I16
N° de cat : CL488-81042
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL488-81042 cible Lamin A/C dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Recombinant |
| Type | Anticorps |
| Immunogène | Lamin A/C Protéine recombinante Ag0408 |
| Nom complet | lamin A/C |
| Masse moléculaire calculée | 65 kDa |
| Poids moléculaire observé | 65-70 kDa |
| Numéro d’acquisition GenBank | BC003162 |
| Symbole du gène | Lamin A/C |
| Identification du gène (NCBI) | 4000 |
| Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Lamin A/C antibody CL488-81042 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
