Anticorps Recombinant de lapin anti-Lamin B1

• Phare
• Validé par KD/KO

Lamin B1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5H10

N° de cat : 80906-1-PBS

Synonymes

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

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Galerie de données de validation

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  WB analysis using 80906-1-RR (same clone as 80906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80906-1-RR (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 80906-1-RR (same clone as 80906-1-PBS)

  WB result of Lamin B1 antibody (80906-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells. This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80906-1-RR (same clone as 80906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80906-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80906-1-RR against Human Lamin B1 were performed. The affinity constant is 13.8 nM.

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Informations sur le produit

80906-1-PBS cible Lamin B1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lamin B1 Protéine recombinante Ag3631
• Nom complet: lamin B1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC012295
• Symbole du gène: Lamin B1
• Identification du gène (NCBI): 4001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

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