Anticorps Monoclonal anti-Lamin B2

Lamin B2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C7B1

N° de cat : 68475-1-PBS

Synonymes

LMNB2, LMN2, Lamin-B2, LAMB2, 2C7B1

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Galerie de données de validation

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  WB analysis using 68475-1-Ig (same clone as 68475-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68475-1-Ig (Lamin B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68475-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68475-1-Ig (same clone as 68475-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (68475-1-Ig, Clone: 2C7B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68475-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68475-1-PBS cible Lamin B2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lamin B2 Protéine recombinante Ag33296
• Nom complet: lamin B2
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC006551
• Symbole du gène: Lamin B2
• Identification du gène (NCBI): 84823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

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