Anticorps Recombinant de lapin anti-Lamin B2

Lamin B2 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230184G4

N° de cat : 82801-3-PBS

Synonymes

LMNB2, LMN2, Lamin-B2, LAMB2, 230184G4

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Galerie de données de validation

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  IF Staining of HepG2 using 82801-3-RR (same clone as 82801-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-3-RR, Clone: 230184G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82801-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82801-3-RR (same clone as 82801-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Lamin B2 Recombinant antibody (82801-3-RR, Clone:230184G4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82801-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82801-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82801-3-RR against Human Lamin B2 were performed. The affinity constant is 0.428 nM.

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Informations sur le produit

82801-3-PBS cible Lamin B2 dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lamin B2 Protéine recombinante Ag1335
• Nom complet: lamin B2
• Masse moléculaire calculée: 68 kDa
• Numéro d’acquisition GenBank: BC006551
• Symbole du gène: Lamin B2
• Identification du gène (NCBI): 84823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

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