Anticorps Recombinant de lapin anti-LCAT

LCAT Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250320E2

N° de cat : 86041-1-PBS

Synonymes

1-alkyl-2-acetylglycerophosphocholine esterase, EC:2.3.1.43, EC:3.1.1.47, Lecithin-cholesterol acyltransferase, PAF acetylhydrolase

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Galerie de données de validation

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  WB analysis using 86041-1-RR (same clone as 86041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86041-1-RR (LCAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86041-1-PBS in a different storage buffer formulation.

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  Sample test of MP02258-1

  The mean LCAT concentration was determined to be 863.3 ng/mL in mouse serum.

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  Cytometric bead array standard curve of MP02258-1

  Cytometric bead array standard curve of MP02258-1, MOUSE LCAT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86041-2-PBS. Detection antibody: 86041-1-PBS. Standard: Eg2756. Range: 0.781-100 ng/mL.

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Informations sur le produit

86041-1-PBS cible LCAT dans les applications de WB, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LCAT Protéine recombinante Eg2756
• Nom complet: lecithin cholesterol acyltransferase
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: NM_008490.2
• Symbole du gène: Lcat
• Identification du gène (NCBI): 16816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454).

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