Anticorps Recombinant de lapin anti-Leptin

Leptin Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240368E6

N° de cat : 83655-3-RR

Synonymes

Lep, OB, 240368E6

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Galerie de données de validation

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  WB analysis of rat adipose using 83655-3-RR

  Rat adipose tissue was subjected to SDS PAGE followed by western blot with 83655-3-RR (Leptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83655-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83655-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83655-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83655-3-RR against Rat Leptin were performed. The affinity constant is 3.54 nM.

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Applications testées

• Résultats positifs en WB: rat adipose tissue,
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83655-3-RR cible Leptin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: leptin
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 15-16 kDa
• Numéro d’acquisition GenBank: NM_013076.3
• Symbole du gène: Leptin
• Identification du gène (NCBI): 25608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

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