Anticorps Recombinant de lapin anti-Lipocalin-2/NGAL

• Phare
• Validé par KD/KO

Lipocalin-2/NGAL Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G1

N° de cat : 82841-1-PBS

Synonymes

LCN2, lipocalin 2, NGAL, Lcn 2, HNL

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Galerie de données de validation

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  WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

  WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR (same clone as 82841-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82841-1-RR (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82841-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82841-1-RR against Human NGAL were performed. The affinity constant is 0.776 nM.

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Informations sur le produit

82841-1-PBS cible Lipocalin-2/NGAL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: lipocalin 2
• Masse moléculaire calculée: 23kd
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: NP_005555.2
• Symbole du gène: NGAL/LCN2
• Identification du gène (NCBI): 3934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

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