Anticorps Recombinant de lapin anti-Lipocalin-2/NGAL

Lipocalin-2/NGAL Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240792C12

N° de cat : 83853-2-PBS

Synonymes

Lcn2, Lipocalin2/NGAL, 240792C12, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein

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Galerie de données de validation

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  WB analysis using 83853-2-RR (same clone as 83853-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83853-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83853-2-RR against Rat Lipocalin-2/Ngal were performed. The affinity constant is 0.195 nM.

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Informations sur le produit

83853-2-PBS cible Lipocalin-2/NGAL dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lipocalin-2/NGAL Protéine recombinante Eg1022
• Nom complet: lipocalin 2
• Masse moléculaire calculée: 22kDa
• Poids moléculaire observé: 22-25 kDa
• Symbole du gène: Lipocalin-2/NGAL
• Identification du gène (NCBI): 170496
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/Ngal.

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