Anticorps Recombinant de lapin anti-Lipocalin-2/NGAL

Lipocalin-2/NGAL Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240792C12

N° de cat : 83853-2-RR

Synonymes

Lcn2, NGAL, Lipocalin-2, lipocalin 2, 240792C12

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Galerie de données de validation

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  WB analysis using 83853-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 83853-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 83853-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83853-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83853-2-RR against Rat Lipocalin-2/Ngal were performed. The affinity constant is 0.195 nM.

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Applications testées

• Résultats positifs en WB: cellules 3T3-L1, tissu pulmonaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83853-2-RR cible Lipocalin-2/NGAL dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: lipocalin 2
• Masse moléculaire calculée: 22kDa
• Poids moléculaire observé: 22-25 kDa
• Symbole du gène: Lipocalin-2/NGAL
• Identification du gène (NCBI): 170496
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/Ngal.

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