Anticorps Polyclonal de lapin anti-Lumican

Lumican Polyclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10677-1-PBS

Synonymes

LUM, SLRR2D, LDC, KSPG lumican

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Galerie de données de validation

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  WB analysis of Jurkat using 10677-1-AP (same clone as 10677-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10677-1-AP (same clone as 10677-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10677-1-AP (same clone as 10677-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 10677-1-AP (same clone as 10677-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Lumican antibody (10677-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10677-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10677-1-PBS cible Lumican dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Lumican Protéine recombinante Ag1106
• Nom complet: lumican
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 50-90 kDa
• Numéro d’acquisition GenBank: BC007038
• Symbole du gène: Lumican
• Identification du gène (NCBI): 4060
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lumican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)

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