Anticorps Recombinant de lapin anti-Lumican

Lumican Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242615H3

N° de cat : 85343-1-RR

Synonymes

LUM, 242615H3, KSPG lumican, LDC, SLRR2D

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Galerie de données de validation

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  WB analysis using 85343-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85343-1-RR (LUM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 85343-1-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 85343-1-RR (LUM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 85343-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85343-1-RR (Lumican antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85343-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85343-1-RR (Lumican antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules MCF-7, cellules Y79, Protéine recombinante
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85343-1-RR cible Lumican dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lumican Protéine recombinante Eg2923
• Nom complet: lumican
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 50-90 kDa
• Numéro d’acquisition GenBank: NM_002345.3
• Symbole du gène: Lumican
• Identification du gène (NCBI): 4060
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lumican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)

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