Anticorps Recombinant de lapin anti-Ly-6C

Ly-6C Recombinant Antibody for FC

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

HK1.4

N° de cat : CL647-65669

Synonymes

Ly6c, HK1.4, Ly 6C, Ly6c1

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using CL647-65669

  1x10^6 mouse bone marrow cells were surface stained with FITC Plus Anti-Mouse CD11b, and 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (CL647-65669, Clone: HK1.4) or CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL647-65669

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (CL647-65669, Clone: HK1.4) (red) or CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65669 cible Ly-6C dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: lymphocyte antigen 6 complex, locus C1
• Numéro d’acquisition GenBank: BC010764
• Symbole du gène: Ly-6C
• Identification du gène (NCBI): 17067
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).

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