Anticorps Monoclonal anti-Ly-6G/Ly-6C (Gr-1)

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(2)

CloneNo.

RB6-8C5

N° de cat : APC-65140

Synonymes

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using APC-65140

  1X10^6 mouse bone marrow cells were surface co-stained with FITC Plus Anti-Mouse CD11b and 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (APC-65140, Clone: RB6-8C5) or 0.25 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

APC-65140 cible Ly-6G/Ly-6C (Gr-1) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Produit contre des granulocytes d'origine murine
• Nom complet: lymphocyte antigen 6 complex, locus G
• Numéro d’acquisition GenBank: X70920
• Symbole du gène: Ly-6G
• Identification du gène (NCBI): 546644
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Publications

Species	Application	Title
mouse	FC	Cell Death Dis; Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.; Authors - Hanyue Chang
mouse	FC	Vet Res; Vimentin affects inflammation and neutrophil recruitment in airway epithelium during Streptococcus suis serotype 2 infection; Authors - Yu Meng

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