Anticorps Monoclonal anti-Ly-6G/Ly-6C (Gr-1)

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

RB6-8C5

N° de cat : PE-65140

Synonymes

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)



Applications testées

Résultats positifs en cytométriecellules médullaires de souris,

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

FCSee 1 publications below

Informations sur le produit

PE-65140 cible Ly-6G/Ly-6C (Gr-1) dans les applications de FC et montre une réactivité avec des échantillons souris

Réactivité souris
Réactivité citéesouris
Hôte / Isotype Rat / IgG2b, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Produit contre des granulocytes d'origine murine
Nom complet lymphocyte antigen 6 complex, locus G
Numéro d’acquisition GenBankX70920
Symbole du gène Ly6g
Identification du gène (NCBI) 546644
Conjugaison PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Protocole

Product Specific Protocols
FC protocol for PE Ly-6G/Ly-6C (Gr-1) antibody PE-65140Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Mol Med

FTY720 attenuates APAP‑induced liver injury via the JAK2/STAT3 signaling pathway.

Authors - Xiangmin He
mouseFC

Cell Death Dis

Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.

Authors - Hanyue Chang

Int J Nanomedicine

Multi-Site Attack, Neutrophil Membrane-Camouflaged Nanomedicine with High Drug Loading for Enhanced Cancer Therapy and Metastasis Inhibition

Authors - Ran Huang