Anticorps Recombinant de lapin anti-Ly-6G

Ly-6G Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242141B11

N° de cat : 98284-1-RR

Synonymes

Ly6g, 242141B11, Ly 6G, Ly-6G.1

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using 98284-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Ly-6G Rabbit RecAb (98284-1-RR, Clone: 242141B11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b (M1/70) (CL647-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98284-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Ly-6G Rabbit RecAb (98284-1-RR, Clone:242141B11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98284-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98284-1-RR against Mouse Ly-6G were performed. The affinity constant is 0.381 nM.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98284-1-RR cible Ly-6G dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Ly-6G Protéine recombinante Eg2609
• Nom complet: lymphocyte antigen 6 complex, locus G
• Masse moléculaire calculée: 14 kDa
• Numéro d’acquisition GenBank: NM_001310438.1
• Symbole du gène: Ly-6G
• Identification du gène (NCBI): 546644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901).

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