Anticorps Monoclonal anti-Lysozyme

Lysozyme Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G5A8

N° de cat : 66456-1-PBS

Synonymes

LYZ, 1,4-beta-N-acetylmuramidase C, 2G5A8, EC:3.2.1.17, Lysozyme C

---

Galerie de données de validation

• 

  WB analysis of human spleen using 66456-1-Ig (same clone as 66456-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66456-1-Ig (same clone as 66456-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 66456-1-Ig (same clone as 66456-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66456-1-Ig (same clone as 66456-1-PBS)

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66456-1-Ig (same clone as 66456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66456-1-Ig (same clone as 66456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66456-1-PBS cible Lysozyme dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lysozyme Protéine recombinante Ag6917
• Nom complet: lysozyme (renal amyloidosis)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC004147
• Symbole du gène: Lysozyme
• Identification du gène (NCBI): 4069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

{{ptg:RelatedPrimaryAntibodies}}