Anticorps Polyclonal de lapin anti-MAD2L1

• Phare
• Validé par KD/KO

MAD2L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(33)

N° de cat : 10337-1-AP

Synonymes

Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2 like protein 1, MAD2, HSMAD2

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Galerie de données de validation

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  WB analysis using 10337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10337-1-AP

  WB result of MAD2L1 antibody (10337-1-AP, 1:500) with si-control and si-MAD2L1 transfected HEK293 cells.

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  WB analysis of rat liver using 10337-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10337-1-AP

  IP result of anti-MAD2L1 (IP:10337-1-AP, 3ug; Detection:10337-1-AP 1:500) with HEK-293 cells lysate 1800ug.

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  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of mouse ovary using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAD2L1 antibody (10337-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 10337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Raji, cellules THP-1, tissu hépatique de rat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu ovarien de souris, tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 7 publications below
• IF: See 7 publications below

Informations sur le produit

10337-1-AP cible MAD2L1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAD2L1 Protéine recombinante Ag0307
• Nom complet: MAD2 mitotic arrest deficient-like 1 (yeast)
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC000356
• Symbole du gène: MAD2L1
• Identification du gène (NCBI): 4085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Publications

Species	Application	Title
	WB	Sci Adv; The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.; Authors - Changyin Zhou
human	WB,IF	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
human	IF	Cell Death Dis; Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma; Authors - Ece Cakiroglu
	IF	J Cell Biol; Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.; Authors - Tianyu Wu
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu
	WB,IHC	Cancer Res; Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.; Authors - He Feng F

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