Anticorps Monoclonal anti-MAD2L1

• Phare
• Validé par KD/KO

MAD2L1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F5F12

N° de cat : 66014-1-PBS

Synonymes

2F5F12, HSMAD2, MAD2, MAD2 like protein 1, MAD2-like protein 1

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  WB result of MAD2L1 antibody (66014-1-Ig, 1:2000) with si-control and si-MAD2L1 transfected HEK293 cells. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66014-1-Ig (same clone as 66014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66014-1-Ig (same clone as 66014-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 66014-1-Ig (same clone as 66014-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66014-1-Ig (same clone as 66014-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66014-1-Ig (same clone as 66014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66014-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66014-1-PBS cible MAD2L1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAD2L1 Protéine recombinante Ag17512
• Nom complet: MAD2 mitotic arrest deficient-like 1 (yeast)
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC000356
• Symbole du gène: MAD2L1
• Identification du gène (NCBI): 4085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

{{ptg:RelatedPrimaryAntibodies}}