Anticorps Polyclonal de lapin anti-MAD2L1BP

MAD2L1BP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 15344-1-AP

Synonymes

p31(comet), MAD2L1-binding protein, KIAA0110, CMT2

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Galerie de données de validation

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  WB analysis using 15344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15344-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15344-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 15344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 15344-1-AP

  Immunofluorescent analysis of A431 cells using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IHC: tissu cutané humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15344-1-AP cible MAD2L1BP dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAD2L1BP Protéine recombinante Ag7550
• Nom complet: MAD2L1 binding protein
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC002904
• Symbole du gène: MAD2L1BP
• Identification du gène (NCBI): 9587
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.

Publications

Species	Application	Title
human	WB,CoIP	Nat Chem; Multi-tiered chemical proteomic maps of tryptoline acrylamide-protein interactions in cancer cells; Authors - Evert Njomen ; KO Validated

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