Anticorps Monoclonal anti-MAdCAM1

MAdCAM1 Monoclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2A12E8

N° de cat : 66594-1-Ig

Synonymes

hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1

---

Galerie de données de validation

• 

  IHC staining of human colon using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:500 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human small intestine using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human appendicitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with .

• 

  IHC staining of human appendicitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with .

• 

  IF Staining of human tonsillitis using 66594-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66594-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu de côlon humain, tissu d'amygdalite humain, tissu d'appendicite humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

66594-1-Ig cible MAdCAM1 dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAdCAM1 Protéine recombinante Ag16184
• Nom complet: mucosal vascular addressin cell adhesion molecule 1
• Masse moléculaire calculée: 406 aa, 43 kDa
• Numéro d’acquisition GenBank: BC142629
• Symbole du gène: MADCAM1
• Identification du gène (NCBI): 8174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

Publications

Species	Application	Title
human	IHC	J Crohns Colitis; Mucosal addressin cell adhesion molecule 1 expression reflects mucosal inflammation and subsequent relapse in patients with ulcerative colitis; Authors - Kazuhiko Uchiyama
human	IHC	Int J Cancer; MAdCAM-1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals; Authors - Naoya Ozawa