Anticorps Polyclonal de lapin anti-MADD

MADD Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29663-1-PBS

Synonymes

MAP kinase-activating death domain protein, KIAA0358, IG20, FLJ36300, FLJ35600

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Galerie de données de validation

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  WB analysis using 29663-1-AP (same clone as 29663-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29663-1-AP (MADD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29663-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 29663-1-AP (same clone as 29663-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29663-1-AP (MADD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29663-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29663-1-PBS cible MADD dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MADD Protéine recombinante Ag31292
• Nom complet: MAP-kinase activating death domain
• Masse moléculaire calculée: 1544 aa, 171 kDa
• Poids moléculaire observé: 200-250 kDa
• Numéro d’acquisition GenBank: BC040484
• Symbole du gène: MADD
• Identification du gène (NCBI): 8567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MADD, MAP kinase-activating death domain protein, belongs to the DENN family, which regulates the Rab family is small GTPases (PMID: 20937701, PMID: 18559336). MADD plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction. MADD is also involved in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PMID: 32761064). KIF1Bβ- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through MADD (PMID: 18849981).

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