Anticorps Polyclonal de lapin anti-MAEA

• Phare
• Validé par KD/KO

MAEA Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (2)

N° de cat : 28363-1-AP

Synonymes

EMLP, EMP, HLC 10, MAEA, PIG5

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Galerie de données de validation

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  WB analysis using 28363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28363-1-AP (MAEA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28363-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28363-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

28363-1-AP cible MAEA dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAEA Protéine recombinante Ag27150
• Nom complet: macrophage erythroblast attacher
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC001225
• Symbole du gène: MAEA
• Identification du gène (NCBI): 10296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).

Publications

Species	Application	Title
human	WB	Cancers (Basel); Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.; Authors - Chunyuan Tang
human	WB	Oncogene; E3 ligase MAEA-mediated ubiquitination and degradation of PHD3 promotes glioblastoma progression; Authors - Peijun Zhou ; KD Validated
human	WB	Chembiochem; Identifying E3 ligase substrates with quantitative degradation proteomics; Authors - Victoria N Jordan ; KO Validated
human	WB,IHC	J Transl Med; LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer; Authors - Yingming Jiang
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi
human,mouse	WB,IHC	Int J Biol Sci; The E3 ubiquitin ligase MAEA promotes macrophage phagocytosis and inhibits gastrointestinal cancer progression by mediating PARP1 ubiquitination and degradation; Authors - Yanchun Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (12-31-2021)

Quite a good quality antibody. KO validated.

• Applications: Western Blot
• Cell Tissue Type: HEK293T

MAEA Antibody Western Blot validation ( dilution) in HEK293T (Cat no:28363-1-AP)

FH

H (Verified Customer) (07-09-2020)

This is very good antibody. MAEA band is very thick and clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

MAEA Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:28363-1-AP)

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