Anticorps Recombinant de lapin anti-MAEA

MAEA Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240097E6

N° de cat : 83267-4-PBS

Synonymes

240097E6, Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP

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Galerie de données de validation

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  WB analysis using 83267-4-RR (same clone as 83267-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83267-4-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00233-4

  Sandwich ELISA standard curve of MP00233-4, Human MAEA Recombinant Matched Antibody Pair - PBS only. 83267-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27150. 83267-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00233-4

  The mean MAEA concentration was determined to be 38.39 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

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  IF Staining of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAEA antibody (83267-4-RR, Clone: 240097E6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-4-RR, Clone:240097E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83267-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83267-4-PBS against Human MAEA was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83267-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83267-4-RR against Human MAEA were performed. The affinity constant is 0.283 nM.

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Informations sur le produit

83267-4-PBS cible MAEA dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAEA Protéine recombinante Ag27150
• Nom complet: macrophage erythroblast attacher
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC001225
• Symbole du gène: MAEA
• Identification du gène (NCBI): 10296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).

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