Anticorps Polyclonal de lapin anti-MAFF

• Phare
• Validé par KD/KO

MAFF Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (2)

N° de cat : 12771-1-AP

Synonymes

hMafF, MAFF, Transcription factor MafF, U MAF

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Galerie de données de validation

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  WB analysis of human lung using 12771-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12771-1-AP (MAFF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of SKOV-3 using 12771-1-AP

  IP result of anti-MAFF (IP:12771-1-AP, 4ug; Detection:12771-1-AP 1:500) with SKOV-3 cells lysate 3200 ug.

• 

  IHC staining of human ovary tumor using 12771-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12771-1-AP (MAFF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 12771-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12771-1-AP (MAFF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12771-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12771-1-AP (MAFF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire humain
• Résultats positifs en IP: cellules SKOV-3,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

12771-1-AP cible MAFF dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAFF Protéine recombinante Ag3460
• Nom complet: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
• Masse moléculaire calculée: 17 aa, 8 kDa
• Poids moléculaire observé: 18-23 kDa
• Numéro d’acquisition GenBank: BC015037
• Symbole du gène: MAFF
• Identification du gène (NCBI): 23764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

Publications

Species	Application	Title
human	WB	Cell; Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress; Authors - Shiyun Cao
human	WB,IHC,IF	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human,mouse	WB,IHC,IF	J Clin Invest; circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.; Authors - Zhenzhen Chen
human	WB	Autophagy; The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3.; Authors - Eutteum Jeong
mouse,rat	WB	Mol Ther Nucleic Acids; miR-320a induces pancreatic β cells dysfunction in diabetes by inhibiting MafF; Authors - Hengzhi Du
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mary Heather Celine (Verified Customer) (09-09-2025)

This antibody produced really clear signal through immunofluorescence staining in young and aged mouse brains. I would want to see if there is a proper control to this antibody given its importance in the aging brain.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse brain

FH

Amy (Verified Customer) (03-18-2024)

Relatively clean bands but not very strong, U2OS lysates 25ug.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: U2OS

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