Anticorps Recombinant de lapin anti-MAFF

MAFF Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242859F9

N° de cat : 85186-3-PBS

Synonymes

V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, U-Maf, U MAF, 242859F9

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Galerie de données de validation

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  WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

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  WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 85186-3-RR (same clone as 85186-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAFF antibody (85186-3-RR, Clone: 242859F9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

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Informations sur le produit

85186-3-PBS cible MAFF dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAFF Protéine recombinante Ag3460
• Nom complet: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
• Masse moléculaire calculée: 17 aa, 8 kDa
• Poids moléculaire observé: 18-23 kDa
• Numéro d’acquisition GenBank: BC015037
• Symbole du gène: MAFF
• Identification du gène (NCBI): 23764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

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