Anticorps Polyclonal de lapin anti-MAFK

MAFK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 33199-1-PBS

Synonymes

Erythroid transcription factor NF-E2 p18 subunit, Transcription factor MafK

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Galerie de données de validation

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  WB analysis using 33199-1-AP (same clone as 33199-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33199-1-AP (MAFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 33199-1-AP (same clone as 33199-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAFK antibody (33199-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

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Informations sur le produit

33199-1-PBS cible MAFK dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAFK Protéine recombinante Ag38230
• Nom complet: v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)
• Masse moléculaire calculée: 18 kDa，156aa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: NM_002360.3
• Symbole du gène: MAFK
• Identification du gène (NCBI): 7975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The small Maf proteins (sMafs) are basic region leucine zipper (bZIP)-type transcription factors. MAFF, MAFG, and MAFK are the three vertebrate sMafs (PMID: 27058431). They act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2, and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PMID: 8932385; 9150357). In cancer cell lines, MAFK promotes epithelial-to-mesenchymal transition (EMT) by downregulating E-cadherin expression through the targeting of transmembrane glycoprotein nonmetastatic B (GPNMB), a potent inducer of EMT (PMID: 28400538; 36061142).

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