Anticorps Monoclonal anti-MAG

MAG Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H9G3

N° de cat : 66709-1-PBS

Synonymes

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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Galerie de données de validation

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  WB analysis of rat brain using 66709-1-Ig (same clone as 66709-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 66709-1-Ig (same clone as 66709-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66709-1-Ig (same clone as 66709-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66709-1-Ig (same clone as 66709-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66709-1-PBS cible MAG dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAG Protéine recombinante Ag6065
• Nom complet: myelin associated glycoprotein
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC053347
• Symbole du gène: MAG
• Identification du gène (NCBI): 4099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

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