Anticorps Recombinant de lapin anti-MAGEA3

MAGEA3 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240435G9

N° de cat : 83480-5-PBS

Synonymes

240435G9, Antigen MZ2 D, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3

---

Galerie de données de validation

• 

  WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 83480-5-RR (same clone as 83480-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MAGEA3 antibody (83480-5-RR, Clone: 240435G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83480-5-RR (same clone as 83480-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 83480-5-RR (same clone as 83480-5-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83480-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83480-5-RR against Human MAGEA3 were performed. The affinity constant is 73.7 pM.

---

Informations sur le produit

83480-5-PBS cible MAGEA3 dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAGEA3 Protéine recombinante Ag0265
• Nom complet: melanoma antigen family A, 3
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC000340
• Symbole du gène: MAGEA3
• Identification du gène (NCBI): 4102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.

{{ptg:RelatedPrimaryAntibodies}}