Anticorps Recombinant de lapin anti-MAGEA3

MAGEA3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240435G9

N° de cat : 83480-5-RR

Synonymes

HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D

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Galerie de données de validation

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  WB analysis using 83480-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83480-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 83480-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MAGEA3 antibody (83480-5-RR, Clone: 240435G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83480-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of Jurkat using 83480-5-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83480-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83480-5-RR against Human MAGEA3 were performed. The affinity constant is 73.7 pM.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules Jurkat, cellules NCI-H1299, cellules SGC-7901
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules HeLa, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83480-5-RR cible MAGEA3 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAGEA3 Protéine recombinante Ag0265
• Nom complet: melanoma antigen family A, 3
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC000340
• Symbole du gène: MAGEA3
• Identification du gène (NCBI): 4102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.

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