Anticorps Polyclonal de lapin anti-MAGEC2

MAGEC2 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10280-1-AP

Synonymes

Cancer/testis antigen 10, CT10, HCA587, MAGE C2 antigen, MAGE E1 antigen, MAGEC2, MAGEE1, melanoma antigen family C, 2, Melanoma associated antigen C2

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Galerie de données de validation

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  WB analysis of HEK-293 using 10280-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10280-1-AP (MAGEC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de mélanome malin humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

10280-1-AP cible MAGEC2 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGEC2 Protéine recombinante Ag0418
• Nom complet: melanoma antigen family C, 2
• Masse moléculaire calculée: 373 aa, 41 kDa
• Numéro d’acquisition GenBank: BC005891
• Symbole du gène: MAGEC2
• Identification du gène (NCBI): 51438
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Melanoma antigen family C, 2(MAGEC2, synonyms: CT10, HCA587, MAGEE1, MGC13377) is related to members of the MAGEC gene family. MAGEC2 is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and other MAGEC genes are clustered on chromosome Xq26-q27.

Publications

Species	Application	Title
human	IHC	PLoS One; γ-Radiation promotes immunological recognition of cancer cells through increased expression of cancer-testis antigens in vitro and in vivo.; Authors - Sharma Anu A
human	IHC	Hum Pathol; Primary mediastinal seminomas: a comprehensive immunohistochemical study with a focus on novel markers.; Authors - Annikka Weissferdt
		Laryngoscope Investig Otolaryngol; The expression of the cancer testis antigen MAGE A4: A favorable prognostic biomarker in salivary gland carcinomas related to low tumor grading.; Authors - Domenic Vital