Anticorps Polyclonal de lapin anti-MAGED1

• Phare
• Validé par KD/KO

MAGED1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 22053-1-AP

Synonymes

DLXIN 1, MAGE D1 antigen, MAGE tumor antigen CCF, MAGED1, melanoma antigen family D, 1, Melanoma associated antigen D1, NRAGE, PP2250

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Galerie de données de validation

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  WB analysis using 22053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22053-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22053-1-AP

  WB result of MAGED1 antibody (22053-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAGED1 transfected A549 cells.

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  WB analysis of mouse placenta using 22053-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22053-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22053-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 22053-1-AP

  IP Result of anti-MAGED1 (IP:22053-1-AP, 4ug; Detection:22053-1-AP 1:1000) with A549 cells lysate 400ug.

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  IHC staining of mouse placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 22053-1-AP

  Immunofluorescent analysis of A549 cells using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 22053-1-AP

  1X10^6 HeLa cells were stained with .2ug MAGED1 antibody (22053-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, tissu placentaire de souris
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu placentaire de souris, tissu cutané humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

22053-1-AP cible MAGED1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGED1 Protéine recombinante Ag17047
• Nom complet: melanoma antigen family D, 1
• Masse moléculaire calculée: 834 aa, 92 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC014070
• Symbole du gène: MAGED1
• Identification du gène (NCBI): 9500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527). Three transcript variants encoding two different isoforms (86kDa and 92 kDa) have been found for the gene of MAGED1.

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice.; Authors - Tingting Qin
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.; Authors - Qiang Lu
human	WB,IHC,IF	Tumour Biol; Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.; Authors - Huandi Zhou ; KD Validated
human	WB,IHC	Gene; NRAGE confers poor prognosis and promotes proliferation, invasion, and chemoresistance in gastric cancer.; Authors - Xiaodi Jiang ; KD Validated