Anticorps Polyclonal de lapin anti-MAGED2

MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15252-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15252-1-AP (same clone as 15252-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15252-1-AP (same clone as 15252-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 15252-1-AP (same clone as 15252-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15252-1-AP (same clone as 15252-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 15252-1-AP (same clone as 15252-1-PBS)

  IP result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15252-1-AP (same clone as 15252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15252-1-AP (same clone as 15252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15252-1-AP (same clone as 15252-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 15252-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15252-1-PBS cible MAGED2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGED2 Protéine recombinante Ag6828
• Nom complet: melanoma antigen family D, 2
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC000304
• Symbole du gène: MAGED2
• Identification du gène (NCBI): 10916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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