Anticorps Polyclonal de lapin anti-MAGED4

MAGED4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16809-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 16809-1-AP (same clone as 16809-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 16809-1-AP (same clone as 16809-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16809-1-PBS cible MAGED4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGED4 Protéine recombinante Ag10329
• Nom complet: melanoma antigen family D, 4
• Masse moléculaire calculée: 741 aa, 81 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC081566
• Symbole du gène: MAGED4
• Identification du gène (NCBI): 728239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition (PMID: 24966945).

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