Anticorps Polyclonal de lapin anti-MAGT1

• Phare
• Validé par KD/KO

MAGT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 17430-1-AP

Synonymes

IAG2, IAP, magnesium transporter 1, MAGT1, OST3B, PRO0756, UNQ628/PRO1244

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Galerie de données de validation

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  WB analysis using 17430-1-AP

  Mouse cerebellum lysates were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17430-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17430-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 17430-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17430-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17430-1-AP (MAGT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17430-1-AP (MAGT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17430-1-AP (MAGT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 17430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 17430-1-AP (MAGT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 17430-1-AP

  Immunofluorescent analysis of HepG2 cells, using 17430-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules COLO 320, cellules HEK-293, tissu cardiaque de souris, tissu de côlon de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral de souris, tissu de cancer du côlon humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IF: See 5 publications below

Informations sur le produit

17430-1-AP cible MAGT1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGT1 Protéine recombinante Ag11496
• Nom complet: magnesium transporter 1
• Masse moléculaire calculée: 335 aa, 38 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: BC060842
• Symbole du gène: MAGT1
• Identification du gène (NCBI): 84061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients'cells by western blot or immunofluorescent cell surface staining. MAGT1 had been detected as 38 kDa (PMID: 18705540) or 45-47 kDa (PMID: 27383987) by western blot.

Publications

Species	Application	Title
human	WB,IF	Nature; Second messenger role for Mg2+ revealed by human T-cell immunodeficiency.; Authors - Li Feng-Yen FY
human	WB	Nature; Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.; Authors - Caleb D Marceau ; KO Validated
rat	WB,IF	Adv Sci (Weinh); A Magnesium-Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration.; Authors - Sihan Lin
rat	WB,IF	Small; Magnetic Field Boosts the Transmembrane Transport Efficiency of Magnesium Ions from PLLA Bone Scaffold; Authors - Zuyun Yan
human	WB, IF	J Cell Biol; Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.; Authors - Natalia A Cherepanova ; KD Validated
human	WB	MBio; Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.; Authors - David L Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Natalia (Verified Customer) (09-26-2019)

To my knowledge, this one is the only one commercially available antibody that works well for WB application to detect MagT1. It cross-reacts with TUSC3 a little, only because these are highly homological proteins.

• Applications: Western Blot, Immunofluorescence,

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