Anticorps Monoclonal anti-MALT1

MALT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

4D3F6

N° de cat : 66225-1-Ig

Synonymes

4D3F6, EC:3.4.22.-, MALT 1, MALT lymphoma-associated translocation, MLT

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Galerie de données de validation

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  WB analysis of HeLa using 66225-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66225-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66225-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66225-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 66225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66225-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66225-1-Ig(MALT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66225-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MALT1 Monoclonal antibody (66225-1-Ig, Clone:4D3F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

66225-1-Ig cible MALT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MALT1 Protéine recombinante Ag20632
• Nom complet: mucosa associated lymphoid tissue lymphoma translocation gene 1
• Masse moléculaire calculée: 824 aa, 92 kDa
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: BC030143
• Symbole du gène: MALT1
• Identification du gène (NCBI): 10892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.

Publications

Species	Application	Title
mouse,human	IHC,CoIP	Cell Biol Toxicol; The COP9 signalosome stabilized MALT1 promotes Non-Small Cell Lung Cancer progression through activation of NF-κB pathway; Authors - Yinghui Wang
human	IHC	Front Cell Dev Biol; Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis.; Authors - Rui Qian

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