Anticorps Polyclonal de lapin anti-MANEA

MANEA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21296-1-PBS

Synonymes

Glycoprotein endo-alpha-1,2-mannosidase, Endomannosidase, Endo-alpha mannosidase, Endo alpha mannosidase, ENDO

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 21296-1-AP (same clone as 21296-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:100 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 21296-1-AP (same clone as 21296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 21296-1-AP (same clone as 21296-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MANEA antibody (21296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21296-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

21296-1-PBS cible MANEA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MANEA Protéine recombinante Ag15847
• Nom complet: mannosidase, endo-alpha
• Masse moléculaire calculée: 462 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC146671
• Symbole du gène: MANEA
• Identification du gène (NCBI): 79694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}