Anticorps Monoclonal anti-MAOA

MAOA Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1A9E4

N° de cat : 68471-1-PBS

Synonymes

1A9E4, Amine oxidase [flavin-containing] A, EC:1.4.3.21, MAO A, MAO-A

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Galerie de données de validation

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  WB analysis using 68471-1-Ig (same clone as 68471-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68471-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 68471-1-Ig (same clone as 68471-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68471-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68471-1-Ig (same clone as 68471-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68471-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68471-1-Ig (same clone as 68471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68471-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68471-1-Ig (same clone as 68471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68471-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68471-1-Ig (same clone as 68471-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MAOA Monoclonal antibody (68471-1-Ig, Clone:1A9E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68471-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68471-1-PBS cible MAOA dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAOA Protéine recombinante Ag30028
• Nom complet: monoamine oxidase A
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC008064
• Symbole du gène: MAOA
• Identification du gène (NCBI): 4128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

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