Anticorps Polyclonal de lapin anti-MAOB

MAOB Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 12602-1-AP

Synonymes

MAO B, MAOB, Monoamine Oxidase B, Monoamine oxidase type B

---

Galerie de données de validation

• 

  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12602-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12602-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12602-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12602-1-AP

  IP result of anti-MAOB(IP:12602-1-AP, 4ug; Detection:12602-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

• 

  IHC staining of human gliomas using 12602-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12602-1-AP (MAOB antibody) at dilution of 1:100 (under 10x lens).

---

Applications testées

• Résultats positifs en WB: cellules L02, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Informations sur le produit

12602-1-AP cible MAOB dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAOB Protéine recombinante Ag3253
• Nom complet: monoamine oxidase B
• Masse moléculaire calculée: 520 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC022494
• Symbole du gène: MAOB
• Identification du gène (NCBI): 4129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position (PMID:3387449).

Publications

Species	Application	Title
mouse	IF	Neuron; Social deprivation induces astrocytic TRPA1-GABA suppression of hippocampal circuits; Authors - Yi-Ting Cheng
mouse	IF	EMBO Rep; Regional heterogeneity of astrocyte morphogenesis dictated by the formin protein, Daam2, modifies circuit function.; Authors - Juyeon Jo
human	WB	Br J Pharmacol; Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission.; Authors - Ji Geng
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo