Anticorps Monoclonal anti-MAOB

MAOB Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B12F3

N° de cat : 66107-1-PBS

Synonymes

2B12F3, Amine oxidase [flavin-containing] B, EC:1.4.3.21, MAO B, MAO-B

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Galerie de données de validation

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  WB analysis of fetal human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66107-1-Ig (same clone as 66107-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  WB analysis using 66107-1-Ig (same clone as 66107-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 66107-1-Ig (same clone as 66107-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 66107-1-Ig (same clone as 66107-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66107-1-PBS cible MAOB dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAOB Protéine recombinante Ag17918
• Nom complet: monoamine oxidase B
• Masse moléculaire calculée: 520 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC022494
• Symbole du gène: MAOB
• Identification du gène (NCBI): 4129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).

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