Anticorps Polyclonal de lapin anti-MAP1B

• Phare
• Validé par KD/KO

MAP1B Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 21633-1-AP

Synonymes

MAP 1B, MAP1B, MAP5

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Galerie de données de validation

• 

  WB analysis of mouse cerebellum using 21633-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21633-1-AP (MAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21633-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21633-1-AP (MAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 21633-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 21633-1-AP (MAP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21633-1-AP (MAP1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 21633-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug MAP1B antibody (21633-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 3 publications below

Informations sur le produit

21633-1-AP cible MAP1B dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP1B Protéine recombinante Ag16255
• Nom complet: microtubule-associated protein 1B
• Masse moléculaire calculée: 2468 aa, 271 kDa
• Poids moléculaire observé: 320 kDa
• Numéro d’acquisition GenBank: BC141853
• Symbole du gène: MAP1B
• Identification du gène (NCBI): 4131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)

Publications

Species	Application	Title
human	WB	EMBO Rep; MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.; Authors - Kohei Arasaki ; KD Validated
mouse	WB	Cereb Cortex; eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.; Authors - Christopher Heise
mouse	IF	Front Cell Neurosci; Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis; Authors - Yingzhen Zhang
mouse	WB	Hum Mol Genet; Microtubule associated protein 1B dysregulates microtubule dynamics and neuronal mitochondrial transport in Spinal Muscular Atrophy.; Authors - Gamze Bora
human	WB	Cell Cycle; Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.; Authors - Junyu Wu
mouse	WB,IF	Neurochem Int; FMRP-absence-induced up-regulation of hypothalamic MAP1B expression decreases AgRP level linking with reduces in food intake and body weight.; Authors - Jing-Yi Long ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Albane (Verified Customer) (02-18-2020)

A lot of bands, none at the correct size

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: SH-SY5Y

MAP1B Antibody Western Blot, validation (1/1000 dilution) in SH-SY5Y (Cat no:21633-1-AP)

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