Anticorps Monoclonal anti-MAP2

MAP2 Monoclonal Antibody for IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C3E6

N° de cat : 67015-1-PBS

Synonymes

1C3E6, MAP 2, MAP-2, MAP2A, MAP2B

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Galerie de données de validation

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  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), GFAP antibody (81063-1-RR, Clone: 4C6, red). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67015-1-Ig (same clone as 67015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594 GFAP antibody (CL594-16825, red), TDP-43 antibody (10782-2-AP, Magenta). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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  FC experiment of Neuro-2a using 67015-1-Ig (same clone as 67015-1-PBS)

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67015-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67015-1-PBS cible MAP2 dans les applications de IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAP2 Protéine recombinante Ag11349
• Nom complet: microtubule-associated protein 2
• Masse moléculaire calculée: 200 kDa
• Numéro d’acquisition GenBank: BC038857
• Symbole du gène: MAP2
• Identification du gène (NCBI): 4133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

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