Anticorps Polyclonal de lapin anti-MAP2K3

• Phare
• Validé par KD/KO

MAP2K3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 13898-1-AP

Synonymes

MEK3, MAPKK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3

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Galerie de données de validation

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  WB analysis using 13898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13898-1-AP (MAP2K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13898-1-AP (MAP2K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules THP-1, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below

Informations sur le produit

13898-1-AP cible MAP2K3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP2K3 Protéine recombinante Ag4861
• Nom complet: mitogen-activated protein kinase kinase 3
• Masse moléculaire calculée: 347 aa, 39 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC032478
• Symbole du gène: MAP2K3
• Identification du gène (NCBI): 5606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP2K3, also named MKK3, is a member of the dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division, and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Identification of MAP Kinase Kinase 3 as a protein target of myricetin in non-small cell lung cancer cells; Authors - Ling Gu ; KD Validated
human	WB	Cell Mol Life Sci; HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress; Authors - Joan Sala-Gaston
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Sci Rep; The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.; Authors - Leonardo Pedrazza ; KD Validated
mouse	WB	Mol Immunol; Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.; Authors - Libo Hou
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang

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