Anticorps Polyclonal de lapin anti-MAP2K4

• Phare
• Validé par KD/KO

MAP2K4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 17340-1-AP

Synonymes

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

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Galerie de données de validation

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  WB analysis using 17340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 17340-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17340-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules RAW 264.7, RAW264.7
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below

Informations sur le produit

17340-1-AP cible MAP2K4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP2K4 Protéine recombinante Ag11376
• Nom complet: mitogen-activated protein kinase kinase 4
• Masse moléculaire calculée: 399 aa, 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC036032
• Symbole du gène: MAP2K4
• Identification du gène (NCBI): 6416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IHC	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu
mouse,rat	WB	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
mouse	WB	J Cell Mol Med; NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.; Authors - Ze-Qing Pu
rat	WB	Cells; Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.; Authors - Elaheh Soleimanpour
human	WB	Int J Biochem Cell Biol; Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.; Authors - Xuechao Wan ; KD Validated
human	WB	Dig Dis Sci; LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.; Authors - Jianhui Liu