Anticorps Monoclonal anti-MAP2K4

MAP2K4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E4C1

N° de cat : 67333-1-PBS

Synonymes

MEK4, 2E4C1, c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC:2.7.12.2

---

Galerie de données de validation

• 

  WB analysis using 67333-1-Ig (same clone as 67333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67333-1-Ig (same clone as 67333-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAP2K4 antibody (67333-1-Ig, Clone: 2E4C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67333-1-PBS cible MAP2K4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAP2K4 Protéine recombinante Ag11348
• Nom complet: mitogen-activated protein kinase kinase 4
• Masse moléculaire calculée: 399 aa, 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC036032
• Symbole du gène: MAP2K4
• Identification du gène (NCBI): 6416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

{{ptg:RelatedPrimaryAntibodies}}