Anticorps Polyclonal de lapin anti-MEK6

• Phare
• Validé par KD/KO

MEK6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 12745-1-AP

Synonymes

MAP2K6, MKK6, Dual specificity mitogen-activated protein kinase kinase 6, EC:2.7.12.2, MAP kinase kinase 6

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Galerie de données de validation

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  WB analysis of HeLa using 12745-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12745-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12745-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12745-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu rénal humain
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

12745-1-AP cible MEK6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEK6 Protéine recombinante Ag3706
• Nom complet: mitogen-activated protein kinase kinase 6
• Masse moléculaire calculée: 334 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC012009
• Symbole du gène: MEK6
• Identification du gène (NCBI): 5608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. MEK6 plays a complex role in cancer, with some evidence showing it can promote tumor growth. Overexpression of MEK6 has been linked to decreased survival in lung adenocarcinoma, breast cancer, and melanoma.

Publications

Species	Application	Title
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Sci Rep; The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.; Authors - Leonardo Pedrazza
	WB	Nat Aging; Skin chronological aging drives age-related bone loss via secretion of cystatin-A; Authors - Wenquan Liang
human	WB	Acta Pharmacol Sin; Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway; Authors - Ji-Xian Xiong ; KD Validated
human	WB	Cell Cycle; SAMHD1, positively regulated by KLF4, suppresses the proliferation of gastric cancer cells through MAPK p38 signaling pathway; Authors - Zhangming Chen
human	WB	Sci Rep; Identification of a novel immune checkpoint-related gene signature predicts prognosis and immunotherapy in breast cancer and experiment verification; Authors - Ke Yin

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