Anticorps Polyclonal de lapin anti-MAP4

• Phare
• Validé par KD/KO

MAP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11229-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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  WB analysis using 11229-1-AP (same clone as 11229-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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  WB analysis of C2C12 using 11229-1-AP (same clone as 11229-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 11229-1-AP (same clone as 11229-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

  IP result of anti-MAP4 (IP:11229-1-AP, 4ug; Detection:11229-1-AP 1:1000) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11229-1-PBS cible MAP4 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP4 Protéine recombinante Ag1741
• Nom complet: microtubule-associated protein 4
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 210-240 kDa
• Numéro d’acquisition GenBank: BC012794
• Symbole du gène: MAP4
• Identification du gène (NCBI): 4134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

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