Anticorps Monoclonal anti-MAP4

MAP4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C1A6

N° de cat : 68271-1-PBS

Synonymes

Microtubule-associated protein 4, MAP-4, MAP 4, 1C1A6

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Galerie de données de validation

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  WB analysis using 68271-1-Ig (same clone as 68271-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68271-1-Ig (MAP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68271-1-Ig (same clone as 68271-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAP4 antibody (68271-1-Ig, Clone: 1C1A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68271-1-PBS cible MAP4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAP4 Protéine recombinante Ag1741
• Nom complet: microtubule-associated protein 4
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 210-240 kDa
• Numéro d’acquisition GenBank: BC012794
• Symbole du gène: MAP4
• Identification du gène (NCBI): 4134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

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