Anticorps Polyclonal de lapin anti-MAP7

MAP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13446-1-PBS

Synonymes

Microtubule-associated protein 7, MAP-7, Epithelial microtubule-associated protein of 115 kDa, Ensconsin, E-MAP-115

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Galerie de données de validation

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  WB analysis of A549 using 13446-1-AP (same clone as 13446-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 13446-1-AP (same clone as 13446-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  IP result of anti-MAP7 (IP:13446-1-AP, 4ug; Detection:13446-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13446-1-AP (same clone as 13446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13446-1-AP (MAP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP7 antibody (13446-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13446-1-AP (same clone as 13446-1-PBS)

  Immunofluorescent analysis of Hela cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13446-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13446-1-PBS cible MAP7 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP7 Protéine recombinante Ag4276
• Nom complet: microtubule-associated protein 7
• Masse moléculaire calculée: 749 aa, 84 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC025777
• Symbole du gène: MAP7
• Identification du gène (NCBI): 9053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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